This dataset contains five files containing data from the Wisconsin Department of Natural Resources shared with the Cornell Wildlife Health Lab (CWHL) at Cornell University for the purpose of the Surveillance Optimization Project for Chronic Wasting Disease (SOP4CWD). The files contain the following information: records in standardized form depicting the results of chronic wasting disease (CWD) testing of white-tailed deer (Odocoileus virginianus) in Wisconsin, US for hunting seasons from 1999-2000 to 2020-21, as completed by wildlife health diagnosticians at (or in partnership with) the Wisconsin Department of Natural Resources; the total number of white-tailed deer (Odocoileus virginianus) legally harvested by hunters by county in Wisconsin, US for hunting seasons from 2014-15 to 2019-20, as recorded by the Wisconsin Department of Natural Resources; the density of white-tailed deer (Odocoileus virginianus) by county in Wisconsin, US at the beginning of the 2019-20 hunting season, as recorded by the Wisconsin Department of Natural Resources; the total number wild cervid meat processors and taxidermists by county in Wisconsin, US for the year 2021, as recorded by the Wisconsin Department of Natural Resources; and the total number of captive cervid facilities by county in Wisconsin, US for the year 2021, as recorded by the Wisconsin Department of Natural Resources.